Gary A. Payne

 
 
 
Professor of Plant Pathology
gary_payne@ncsu.edu

Department of Plant Pathology,
Box 7567
223 Partners III,
North Carolina State University,
Raleigh, NC 27695-7567
Phone: 919.515.6994 Fax: 919.513.0024

Research: 

Visit my lab

Genomic approaches and field studies are being used in my program to improve food safety and to understand fungal biology and secondary metabolism. Gene expression profiling is being used to characterize the regulatory circuits in Aspergillus flavus governing aflatoxin biosynthesis and to elucidate the molecular response in maize kernels to Aspergilus flavus infection.. Field and laboratory studies are focused on understanding the ecology of Aspergillus flavus and Fusarium verticillioides and the inheritance of resistance to the fungi. Maize seed proteins inhibitory to mycotoxin-producing fungi are being isolated and characterized to better understand resistance mechanisms in seeds.

Dr Gary A. Payne
William Neal Reynolds Professor

 

B.S., North Carolina State University
M.S., Cornell University
Ph.D., Cornell University
Research Associate, Laboratory of Agricultural Biochemistry, Lincoln, NE

Teaching: 
 
PP 707 Spring Semester
Fundamental concepts and current status of research on the physiology, biochemistry and molecular biology of host-pathogen interactions. Topics include recognition, penetration and colonization, pathogenicity and virulence determinants, resistance mechanisms, signal  transduction, programmed cell death, and other current topics. Information presented in context of viral-, bacterial-, fungal-and nematode-plant interactions.
 
PP 730 Fall Semester

Basic concepts of genetics, ecology, and physiology of fungi, with emphasis on saprophytic and plant and animal pathogenic mycelial fungi. Current literature on evolution, cell structure, growth and development, gene expression, metabolism, sexual and asexual reproduction and incompatibility systems. 

Selected Publications: 

  • Brittiney N. Reese, Gary A. Payne, Dahlia M. Nielsen, and Charles P. Woloshuk. 2011.  Gene Expression profile and response to maize kernels by Aspergillus flavus. Phytopathology 101:797-804.

  • Wu, F., Bhatnagar, D., Bui-Klimke, T., Carbone, I., Hellmich, R., Munkvold, G., Paul, P., Payne, G., Takle, E. 2011. Climate Change Impacts on Mycotoxin Risks in US Maize. World Mycotoxin Journal. 4: 79-93.

  • Eller, M.S., Payne, G.A., and Holland, J.B. 2010.  Selection for reduced fusarium ear rot and fumonisin content in advanced backcross maize lines and their topcross hybrids.  Crop Science. 50:2249–2260.

  • Chang, K.Y., Georgianna, D.R., Heber, S., Payne, G.A., and Muddiman, D.C. 2010. Detection of alternative splice variants at the proteome level in Aspergillus flavus. Journal of Proteome Research. 9: 1209-1217.

  • Nicholson, M.J., Koulman, A., Monahan, B. J., Pritchard, B.L., Payne, G.A. and Scott, Barry. 2009. Identification of two aflatrem biosynthesis gene loci in Aspergillus flavus and A. oryzae and metabolic engineering of Penicillium paxilli to elucidate their function. Applied and Environmental Microbiology.75:7469-7481.

  • Georgianna, D.G., Fedorova, N.D.,Burroughs, J. L, Dolezal, A.L, Bok, J., Horowitz-Brown, S., Woloshuk, C. P., Yu, J. Keller, N.P., and Payne, G.A. 2010. Beyond aflatoxin: four distinct expression patterns and functional roles associated with Aspergillus flavussecondary metabolism gene clusters. Molecular Plant Pathology 11:213-226.

  • Smith, C.A, Robertson, D. Pritchard, B., Nielsen, D. M., Brown, D. Dean, R.A. and Payne, G.A. 2008. The effect of temperature on Natural Antisense Transcript (NAT) expression in Aspergillus flavus. Current Genetics 54:241-269.

  • Johnson, D. N., Egner, P. A., Obrian, G., Glassbrook, N., Roebuck, B. D., Sutter, T. R., Payne, G. A., Kensler, T. W., and Groopman, J. D. 2008. Quantification of urinary aflatoxin B-1 dialdehyde metabolites formed by aflatoxin aldehyde reductase using isotope dilution tandem mass spectrometry. Chemical Research in Toxicology 21:752-760.

  • Collier, T. S., Hawkridge, A. M., Georgianna, D. R., Payne, G. A., and Muddiman, D. C. 2008. Top-down identification and quantification of stable isotope labeled proteins from Aspergillusflavususing online nano-flow reversed-phase liquid chromatography coupled to a LTQ-FTICR mass spectrometer. Analytical Chemistry 80:4994-5001.

  • Georgianna, D.R., Hawkridge, A.M. Muddiman, D.C., and Payne, G.A. 2008.  Temperature dependent regulation of Proteins in Aspergillusflavus: Whole Organism Stable Isotope Labeling by Amino Acids. Journal of Proteome Research 7:2973-2979.

  • Yu, J.J., Ronning, C. M., Wilkinson, J.R., Campbell, B.C., Payne, G. A., Bhatnagar, D., Cleveland, T.E.  and Nierman, W. C.  2007. Gene profiling for studying the mechanism of aflatoxin biosynthesis in Aspergillus flavusand A. parasiticus. Food Additives and Contaminants 24:1035-1042.

  • Du, W., OBrian, G.R., and Payne, G. A. 2007. Function and regulation of aflJ in the accumulation of aflatoxin early pathway intermediate in Aspergillus flavus. Food Additives and Contaminants 24:1043-1050 2007.

  • Cary, J. W., OBrian, G. R., Nielsen, D.M., Nierman, W., Harris-Coward, P., Yu, J., Bhatnagar, D.,  Cleveland, T. E., Payne, G. A., Calvo, A. M. 2007.  Elucidation of veA-dependent genes associated with aflatoxin and sclerotial production in Aspergillus flavusby functional genomics.  Applied Microbiology and Biotechnology 76: 1107-1118.

  • Smith, C. A., Woloshuk, C. P., Robertson, D. and Payne, G. A. 2007. Silencing of the Aflatoxin Gene Cluster in a Diploid Strain of Aspergillus flavus is Suppressed by Ectopic aflRExpression.  Genetics 179:1-10.

  • OBrian, G.R., Georgianna, D., Wilkinson, J., Yu, J., Abbas, H., Bhatnagar, D., Cleveland, T. E., Nierman, W., and Payne, G. A. 2007. The effect of elevated temperature on gene transcription and aflatoxin biosynthesis. Mycologia 99: 232-239.

  • Robertson-Hoyt, L.A., Kleinschmidt, C.E., White, D.G., Payne, G.A. Maragos, C.M., and Holland, J.B. 2007. Relationships of resistance to fusariumear rot and fumonisin contamination with agronomic performance of maize. Crop Sci. 47:1770-1778

  • Robertson-Hoyt, L.A., Betran, J., Payne, G.A., White, D. G., Isakeit, T., Maragos, C. M., Molnar, T.L., and Holland, J. B. 2007. Relationships among resistances to Fusarium and Aspergillus ear rots and contamination by fumonisin and aflatoxin in maize.  Phytopathology 97:311-17.

  • Starr, M.R., Robertson-Hoyt, L.A., Payne, G.A., and Holland, J. B. 2006.  Improving resistance to fumonisin contamination in maize.  Illinois Breeding School. p. 83-92.

  • Payne, G.A., Nierman, W.C., Wortman, J.R., Pritchard, B.L., Brown, D. Dean, R.A., Bhatnagar, D., Cleveland, T.E., Machida, M. and Yu, J.J. 2006.  Whole genome comparison of Aspergillus flavusand A. oryzae.  Medical Mycology. 44:S9-S11

  • Johansson, A. S., Whitaker, T. B., Hagler, W. M., Bowman, D. T., Slate, A. B., Payne, G. 2006. Predicting aflatoxin and fumonisin in shelled corn lots using poor-quality grade components. Journal of AOAC international, 89(2), 433-440.

  • Robertson, L. A., Jines, M.P., Balint-Kurti, P. J., Kleinschmidt, C. E., White, D. G., Payne, G. A., Maragos, C. M., Molnár, T. L. and Holland. J. B. 2006. QTL mapping for Fusarium ear rot and fumonisin contamination resistance in two populations of maize (Zea mays). Crop Science 46:1734-1743.

  • Price, M. S., J. Yu, W. C. Nierman, H. S. Kim, B. Pritchard, C. A. Jacobus, D. Bhatnagar, T. E. Cleveland, and G. A. Payne. 2006. The aflatoxin pathway regulator AflR induces gene transcription inside and outside of the aflatoxin biosynthetic cluster. FEMS Microbiology Letters 255:275-9.

  • Payne, G.A., Nierman, W.C., Wortman, J.R., Pritchard, B.L., Brown, D. Dean, R.A., Bhatnagar, D., Cleveland, T.E., Machida, M. and Yu, J.J. 2006.  Whole genome comparison of Aspergillus flavusand A. oryzae.  Medical Mycology. 44:S9-S11.

  • Robertson, L.A., Kleinschmidt, C.E., White, D.G., Payne, G.A., Maragos, C.M., and Holland, J.B. 2005. Heritabilities and correlations of Fusarium ear rot resistance and fumonisin contamination resistance in two maize populations. Crop Science 46:353-361

  • Price, M. S., Conners, S. B., Tachdjian, S., Kelly, R. M. and Payne, G. A. 2005.Aflatoxin conducive and non-conducive growth conditions reveal new gene associations with aflatoxin production. Fungal Genetics and Biology 42:506-518.

  • Xue, H. Q., T. G. Isleib, G. A. Payne, W. F. Novitzky, and G. OBrian. 2005. Aflatoxin Production in Peanut Lines Selected To Represent a Range of Linoleic Acid Concentrations. Journal of Food Protection 68:126-132.

  • Yu, J. J., Chang, P.K. Ehrlich, K.C., Cary. J. W., Bhatnagar, D. Cleveland, T. E. Payne, G. A., Linz, J. E., Woloshuk, C. P. and Bennett, J. W. 2004. Clustered pathway genes in aflatoxin biosynthesis. Applied and Environmental Microbiology 70: 1253-1262.

  • Pirttilä, A. M., McIntyre, L. M., Payne, G. A., and Woloshuk, C. P. 2004. Expression profile analysis of wild-type and fcc1 mutant strains of Fusarium verticillioidesduring fumonisin biosynthesis. Fungal Genetics and Biology 41:647–656

  • Bush, B. J., Carson, M. L., Cubeta, M. A., Hagler, W. M., and Payne, G. A. 2004. Infection and Fumonisin Production by Fusariumverticillioides in developing maize kernels. Phytopathology 94:88-93.

  • Moore, K. G., Price, M. S., Boston, R. S., Weissinger, A.K., and Payne, G. A. 2004. A Chitinase from Tex6 Maize Kernels Inhibits Growth of Aspergillusflavus. Phytopathology 94:82-87.

  • Brown, R. L., C. S. Brown-Jenco, Bhatnagar, D., and Payne, G.A. 2003. Construction and preliminary evaluation of an Aspergillusflavusreporter gene construct as a potential tool for screening aflatoxin resistance. Journal of Food Protection. 66:1927-1931.

  • OBrian, G. O., Fakhoury, A. M. and Payne, G.A. 2003.Identification of genes differentially expressed during aflatoxin biosynthesis in Aspergillus flavusand Aspergillus parasiticus. Fungal Genetics and Biology. 2003. 39:118-27.

  • Price, M. S., J. J. Classen, and G. A. Payne. 2001. Aspergillusnigerabsorbs copper and zinc from swine wastewater. J. Bioresour. Technol. 77:41-49.

  • Nielsen, K, G. A. Payne and R. S. Boston. 2001. Maize ribosome inactivating protein inhibits normal development of Aspergillusnidulansand Aspergillusflavus. Mol. Plant Microb. Int. 14:164-172.

Research review articles in peer-reviewed journals

  • Georgianna, R and Payne, G. A. 2006. Regulation of aflatoxin biosythesis. Fungal Genetics and Biology. 46:113-125.

  • Payne, G. A., A.M. Fakhoury, K. A. Scheidegger, M.S. Price. 2004. Genomic analysis of aflatoxin biosynthesis. Pages 89-97, in New Horizon of Mycotoxicology for Assuring Food Safety. Proceedings of the International Symposium of Mycotoxicology in Kagawa 2003, T. Yoshizawa, Ed. Tokyo, Japan.

  • Scheidegger, K. A. and Payne, G. A. 2003. Unlocking the secrets behind secondary metabolism: a review of Aspergillus flavus from pathogenicity to functional genomics. J. of Toxicology 22:423-459.

Books and Book Chapters

  • Yu, J., Nierman, W. C., Bennett, J. W., Cleveland, T. E. Campbell, C. C., Bhatnagar, D., Dean, R. A., and Payne, G. A. 2010.  Genetics and Genomics of Aspergillus flavus. In: Rai, M. K. and Kovics, G. (ed). Progress in Mycology. Springer Verlag. Chapter 2. Pages 51-73.

  • Payne, G. A. Genomics of Aspergillus flavus. 2007. In, The Aspergilli: Genomics, Medical Applications, Biotechnology, and Research Methods. Osmoni and Goldman CRC Press.

  • Payne, G. A. and Cubeta, M. A. 2005. Biology and detection of fungal pathogens of humans and plants. In Microbial Forensics, Eds. R.G. Breeze, B. Budowle, and S. E. Schutzer. Elsevier Academic Press. New York.

  • Robertson, L.A., Payne, G.A., and Holland, J.B. 2005. Marker Assisted Breeding for Host Resistance to Mycotoxin Contamination. Aflatoxin and Food Safety, edited by H. Abbas. CRC Press.

  • Price, M. S. and Payne, G. A. 2004. Genomics and gene regulation of the aflatoxin biosynthetic pathway. p.387-404. In Z. An (ed.), Handbook of Industrial Mycology. Marcel Dekker, Inc., New York.

  • Bhatnagar, D., G. A. Payne, T. E. Cleveland, and J. F. Robens. 2004. Mycotoxins: Current Issues in USA. Pages 17-47, in Meeting the Mycotoxin Menace, Eds. H. Barug, H. P. van Egmond, R. Lopez-Garcia, W. A. van Osenbruggen, and A. Visconti. Wageningen Academic Publishers, Netherlands.

  • Robertson, L.A., Payne, G.A., and Holland, J.B. 2005. Marker Assisted Breeding for Host Resistance to Mycotoxin Contamination. Aflatoxin and Food Safety, edited by H. Abbas. CRC Press.

  • Fakhoury, A. K. and Payne, G. A. 2003. Genomics of filamentous fungi: A general overview. In D. K. Arora, ed. Handbook of Fungal Biotechnology. 2nd Ed. Volume 20 of the Mycology Series.

  • Wu, F., Bhatnagar, D., Bui-Klimke, T., Carbone, I., Hellmich, R., Munkvold, G., Paul, P., Payne, G., Takle, E. 2011. Climate Change Impacts on Mycotoxin Risks in US Maize. World Mycotoxin Journal. 4: 79-93.

  • Richard, J. L. and G. A. Payne. 2003. Eds., Mycotoxins: Risks in Plant, Animal, and Human Systems. Task Force Report 139. Council for Agricultural Science and Technology. Ames, Iowa. 199 pages.